Figure 1.

Transfection of VSMCs and ECs with MARCKS siRNA silences MARCKS mRNA and protein expression. Transfection of VSMCs (A) and ECs (B) with MARCKS siRNA reduced MARCKS mRNA levels at the end of the transfection period (qRT-PCR analysis). Complete MARCKS knockdown was detected at the protein level by Western blot analysis 3 days after transfection with 100 nmol/L MARCKS siRNA (MKS) in VSMCs (A) and 25 nmol/L MARCKS siRNA in ECs (B). qRT-PCR data represent averages of 3 independent experiments. Western blots correspond to one representative experiment of 3 performed. NT, no treatment; Con, control siRNA. *P < 0.05 vs. control siRNA.