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. 2009 Feb;23(2):557–564. doi: 10.1096/fj.08-114173

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Figure 6. — MARCKS silencing inhibits VSMC proliferation and neointimal formation in cultured human saphenous vein. A) IHC staining for BrdU revealed a reduction in proliferating nuclei (brown nuclei, arrows) measured in vein segments incubated with BrdU after transfection with MARCKS siRNA (right panel) vs. control siRNA (left panel). Micrographs (×400) correspond to one representative experiment of 4 performed. B) Intimal thickness measured in VVG-stained cross sections of transfected vein segments was reduced in patient-matched vein segments transfected with MARCKS siRNA (right panel) vs. control siRNA (left panel). Arrowheads indicate the internal elastic lamina. Micrographs (×400)correspond to one representative experiment of 5 performed.