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. Author manuscript; available in PMC: 2009 Sep 5.
Published in final edited form as: J Mol Biol. 2008 May 29;381(3):581–593. doi: 10.1016/j.jmb.2008.05.049

Figure 4. The GTP-independent complex is stabilized by the 4.5S RNA.

Figure 4

(a) Spectrum of the GTP-independent complex in the absence of 4.5S RNA. The experiment setup is the same as in Fig. 1c except that the 4.5S RNA was not included. (b) Formation of the GTP-independent complex was monitored in the presence (●) and absence (■) of the 4.5S RNA. (c and d) The time course for formation of the GTP-dependent complex was monitored in the presence (c) and absence (d) of 4.5S RNA. In (c), 0.5 µM SRP and 2 µM SR were used. In (d), 2 µM Ffh and 10 µM SR were used to obtain a faster reaction rate. Note the difference in time scales in (c) and (d).