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. Author manuscript; available in PMC: 2009 Sep 5.
Published in final edited form as: J Mol Biol. 2008 May 29;381(3):581–593. doi: 10.1016/j.jmb.2008.05.049

Figure 6. FRET measurement shows the deficiency in SRP-SR complex formation caused by 4.5S RNA tetraloop mutants.

Figure 6

(a) Time course for formation of the GTP-dependent complex in the presence of different RNA mutants. The inset shows the data over a longer time course with the UUCG mutant (time scale in minutes). 0.5 µM SRP (2 µM SRP for UUCG mutant) and 2 µM SR (10 µM SR for UUCG) were used in the experiment in the presence of 100 µM GppNHp. (b) FRET measurement of the extent of formation of the GTP-independent complex with various 4.5S RNA mutants. 4 µM SRP and 16 µM SR were incubated without GppNHp.