TABLE 2. Analysis of serum neutralization mechanism.

Neutralization activity of various plasma against JR-FL-based VLPs was analyzed in various formats. As in Table 1, neutralization formats are organized in columns. IC50 data are given as reciprocal plasma dilutions. Neutralization has been color-coded in a similar manner to Table 1. N.D.=not done. JR-FL gp120 ELISA titers for each serum against gp120 are shown. IC50 data not shown in the table for plasmas N308, A62, L909, and TN15 were 4,000, 300, 170 and 67, respectively against gp160WT-VLPs in the standard format and 15,000, 3,000, 150 and 99 against gp160ΔCT WT-VLPs in the standard format.

		column	1	2	3
		format	standard	post-CD4	post-CD4/CCR5
		WT/SOS	WT		SOS
IC50 titer:	human plasma	gp120 titer
<50	N308	250,000	9,000	240,000	<40
50-300	A62	100,000	2,000	89,600	<40
300-1000	#11H	500,000	1,000	90,000	<40
1000-3000	L92	1,000,000	400	500,000	<40
>3000	L909	1,500,000	320	309,000	<40
	R2	600,000	150	500,000	<40
	739	80,000	100	200,000	<40
	TN15	603,000	64	143,000	40
	J864	248,000	50	84,000	<40
	N160	500,000	<40	160,000	<40
	K370	381,000	<40	100,000	<40
	TN11	119,000	<40	19,000	<40
	L503	612,000	<40	162,000	<40
	HIV-	<300	<40	250	<40
	spiked human plasma
	J864	248,000	50	84,000	<40
	J864+b12	N.D.	950	90,000	<10
	J864+2G12	N.D.	120	80,000	<10
	J864+2F5	N.D.	110	82,000	71