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. Author manuscript; available in PMC: 2009 Jan 26.
Published in final edited form as: J Biol Chem. 2007 Jul 20;282(38):27736–27743. doi: 10.1074/jbc.M703788200

FIGURE 2.

FIGURE 2

a−d, light scattering was used to compare the assembly of 10 μm MtbFtsZ before and after labeling with fluorophores. a, unlabeled; b, fluorescein-labeled; c, TMR-labeled; d, a mixture of 5 μm MtbFtsZ-fluorescein and 5 μm MtbFtsZ-TMR. Curve e shows this mixture assayed for assembly by FRET. Light-scattering assays were excited and detected at 350 nm; the FRET assay was excited at 450 nm and detected at 515 nm (assembly is measured by the decrease in donor emission at 515 nm). The FRET signal was normalized to the light-scattering signal. Both light-scattering and fluorescence measurement started immediately after adding GTP.