. Author manuscript; available in PMC: 2009 Jan 26.

Published in final edited form as: J Biol Chem. 2007 Jul 20;282(38):27736–27743. doi: 10.1074/jbc.M703788200

TABLE 1. Summary of GDP-induced disassembly, subunit turnover, and GTPase at steady state.

All measurements were done at room temperature, so the GTPase of EcFtsZ is 2−3 times lower than the values we reported previously at 37 °C (12).

pH	GDP disassembly t_½	Turnover t_½	GTPase	1/GTPase
	s	s	GTP FtsZ⁻¹ min⁻¹	s
*E. coli*
6.5	6	8	4 ± 2	15
7.7	4	5.5	7 ± 1	8.6
*M. tuberculosis*
6.5	131	(163)^a	(0.08)^a ± 0.02	(750)^a
6.9	51	(80)^a	(0.26 ± 0.05)^a	(230)^a
7.7	28	42	0.8 ± 0.2	75

Turnover numbers for MtbFtsZ at pH 6.5 and 6.9 are in parentheses because turnover was limited in extent; 1/GTPase numbers are exaggerated for these cases because they are based on total FtsZ, including the subunits that are not turning over.