Table 1.
Quantification of wound healing in stably transfected MCF-7-Ago2 cells
| Cell type | Time point | Width of the wound (mm)
|
Total wound closure (%) | |
|---|---|---|---|---|
| Average (n = 6) | ± sem | |||
| MDA-MB-231 | 0 h | 188.98 | 6.528 | |
| 8 h | 124.97 | 7.061 | ||
| 16 h | 90.42 | 7.315 | ||
| 24 h | 51.31 | 5.639 | 72.8 | |
| MCF-7-Par | 0 h | 189.48 | 10.52 | |
| 8 h | 184.41 | 11.63 | ||
| 16 h | 174.24 | 10.29 | ||
| 24 h | 162.81 | 15.01 | 14.1 | |
| MCF-7-empty | 0 h | 191.52 | 8.001 | |
| 8 h | 184.66 | 4.191 | ||
| 16 h | 172.47 | 2.845 | ||
| 24 h | 157.23 | 2.642 | 17.9 | |
| MCF-7-Ago2-WT | 0 h | 189.48 | 12.42 | |
| 8 h | 160.27 | 5.537 | ||
| 16 h | 137.92 | 4.267 | ||
| 24 h | 121.49 | 4.343 | 35.9 | |
| MCF-7-Ago2_167C | 0 h | 185.32 | 11.13 | |
| 8 h | 168.52 | 4.325 | ||
| 16 h | 161.73 | 3.451 | ||
| 24 h | 155.12 | 3.327 | 16.3 | |
| MCF-7-Ago2_408C | 0 h | 190.37 | 10.17 | |
| 8 h | 175.19 | 8.924 | ||
| 16 h | 165.23 | 6.174 | ||
| 24 h | 156.48 | 5.052 | 17.8 | |
The table depicts ″total wound closure″ for each cell type, as determined by relative distance encroached upon by invasive cells after 24 h. Each assay was performed three times with duplicates within each experiment and reported as the mean ± sem. Values from various Ago2 transfectants are representative of three pools of stable transfectants with equivalent WT or mutant Ago2 expression. At 16 h, wound closure was significantly (P < 0.05) greater in MCF-7-Ago2-WT as compared with MCF-7-Par cells.