Table 2.
Respiratory parameters, ADP/O ratios, and ATP production rates (mean ± sem) in mitochondria isolated from the tissues indicated of control and STZ-diabetic group III (2-month diabetic) rats
| Gastrocnemius
|
Heart
|
Liver
|
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Succinate
|
Glut/mal
|
Succinate
|
Glut/mal
|
Succinate
|
Glut/mal
|
|||||||
| Ctrl | STZ-D | Ctrl | STZ-D | Ctrl | STZ-D | Ctrl | STZ-D | Ctrl | STZ-D | Ctrl | STZ-D | |
| State 4 respiration (nmol O/min/mg) | 103 ± 10 | 90 ± 11 | 67 ± 6 | 64 ± 9 | 203 ± 8 | 165 ± 14a | 81 ± 6 | 69 ± 5 | 72 ± 3 | 71 ± 2 | 31 ± 4 | 28 ± 2 |
| State 3 respiration (nmol O/min · mg) | 252 ± 22 | 243 ± 30 | 463 ± 37 | 431 ± 48 | 311 ± 10 | 284 ± 20 | 402 ± 39 | 395 ± 28 | 264 ± 15 | 236 ± 13 | 177 ± 18 | 171 ± 12 |
| FCCP respiration (nmol O/min · mg) | 231 ± 28 | 210 ± 26 | 875 ± 99 | 697 ± 109 | 333 ± 9 | 288 ± 17a | 576 ± 129 | 458 ± 71 | 495 ± 49 | 414 ± 30 | 417 ± 54 | 405 ± 30 |
| RCR | 2.62 ± 0.28 | 2.71 ± 0.18 | 7.29 ± 1.03 | 7.21 ± 0.75 | 1.54 ± 0.05 | 1.75 ± 0.13 | 5.15 ± 0.55 | 5.87 ± 0.43 | 3.66 ± 0.14 | 3.38 ± 0.22 | 6.12 ± 0.93 | 6.37 ± 0.79 |
| ADP/O | 3.83 ± 0.42 | 3.79 ± 0.34 | 1.38 ± 0.09 | 1.54 ± 0.44 | 3.00 ± 0.05 | 3.11 ± 0.21 | 0.96 ± 0.08 | 1.15 ± 0.09 | 0.96 ± 0.06 | 1.00 ± 0.06 | 1.50 ± 0.14 | 1.56 ± 0.14 |
| MAPR (nmol/min/mg) | 203 ± 12 | 218 ± 27 | 181 ± 25 | 226 ± 31 | 263 ± 38 | 291 ± 31 | 226 ± 23 | 293 ± 45 | ND | ND | ND | ND |
Ctrl, Control; Glut/mal, Glutamate/malate; MAPR, mean ATP production rate; ND, not determined; RCR, respiratory control ratio (state 3/state 4 respiration); STZ-D, STZ-diabetic.
a
P < 0.05; n = 6–7 rats per group. Significantly different values are in bold.