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Effect of KCl on RAD51 binding to dsDNA: gel shift assay. Supercoiled φX174 DNA (30 μM) was incubated with RAD51 (3 μM) with ATP (1 mM) in binding buffer R at 37°C for 12 minutes in presence of increasing concentrations of KCl [0 mM, 25 mM, 50 mM, 100 mM, 140 mM & 200 mM] (lanes 2–7), followed by gel-shift analyses (Methods). Lane 1 represents no protein control.
