Figure 3.

2-D reference map of total proteins extracted from developing white lupin roots. One thousand two hundred μg proteins were separated by IEF (pH 3–11) and SDS-PAGE (12% vertical gel) and stained with Coomassie blue R-250. One hundred and ninety proteins were selected (indicated by numbered spots) across the PI and molecular mass range, in-gel digested with trypsin and subjected to MALDI-TOF peptide mass fingerprinting.