Figure 2.

Virus replication and DNA synthesis in BS-C-1 cells. Confluent 6 well plates of BS-C-1 cells were infected with WR and recombinant viruses at a multiplicity of 5. The infected cells were harvested at the indicated hours post infection (hpi) to determine virus titers and viral DNA. (A) Virus titers were determined on BS-C-1 cells and plotted as PFU/ml. All experiments were carried out in triplicate. Average titers are shown; bars representing standard error of the mean could not be printed because of their very close spacing. (B) Viral DNA was determined by blot hybridization and quantified with a phosphorImager. Experiment represents average of duplicate experiments.