Figure 1.

Expression levels of MMPs (A) and their inhibitors (B) by qRT-PCR using total RNA from a panel of five human breast cancer cell lines, displaying increased degrees of invasiveness and metastatic potential, cultured on different substrates (plastic or Matrigel). GAPDH was used to normalize the values. Results are presented as MEANS ± SD of two independent experiments. P values were derived from One-way analysis of variance and Tukey-Kramer test. *, p < 0.05; **, p < 0.01 and ***p < 0.001, all versus control (MCF-7).