Table 4.
Summary of LOH analysis of FAT gene locus in astrocytic tumors (20 grade II and 20 grade IV) by RFLP using SNP markers.
| Summary of LOH detected with each SNP markers | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| SNP-Markers | rs22 76930 | rs37 33414 | rs45 5600 | rs21 3090 | rs45 0320 | rs76 63350 | rs11 93110 | rs12 98865 | |
| Informative samples | Grade II | 0/20 (0%) | 15/20 (75%) | 7/20 (35%) | 9/20 (45%) | 12/20 (60%) | 7/20 (35%) | 2/20 (10%) | 0/20 (0%) |
| Grade IV | 0/20 (0%) | 8/20 (40%) | 6/20 (30%) | 6/20 (30%) | 10/20 (50%) | 0/20 (0%) | 2/20 (10%) | 1/20 (5%) | |
| LOH per informative samples | Grade II | 0/20 (0%) | 2/15 (13.33%) | 2/7 (28.57%) | 3/9 (33.33%) | 4/12 (33.33%) | 0/7 (0%) |
0/2 (0%) |
0/0 (0%) |
| Grade IV | 0/20 (0%) | 3/8 (37.5%) | 2/6 (33.33%) | 3/6 (50%) | 5/10 (50%) | 0/0 (0%) |
1/2 (50%) | 1/1 (100%) | |
|
Total LOH (Grade II & IV) |
0/20 (0%) |
5/23 21.73% |
4/13 30.73% |
6/15 40% |
9/22 40.90% |
0/7 (0%) |
1/4 25% |
1/1 100% | |
| LOH per informative samples | |||||||||
| Samples | Grade II (N = 20) | Grade IV (N = 20) | Grade II&IV (N = 40) | ||||||
|
Overall SNP LOH per informative samples |
8/19* (42.10%) |
11/19* (57.89%) |
19/38 (50%) |
||||||
And SNP LOH detected per informative samples (Grade II and IV) with all SNP markers.
* In both grade II and grade IV one sample each were uninformative with all the SNP markers used.