Table 1.

Primers and RT-PCR conditions

Primers employed in the RT-PCR				RT-PCRs performed
Primer	Sequence (5'->3')	GLB1Exona	Transcript specificity	Pairs of primers employed	AT (°C)
C1F	ACTGCAGAGCCGGGAGGCTGGT	1	EBP/β-Gal	C1F/EBPR	58
C3R	CCAAAGTGACCTTTCCATATG	11	EBP/β-Gal	EBPF/C3R	56.5
EBPF	GGCTGAACGCCATCCAGACATT	2–5	EBP	T7/SP6	55
EBPR	TGATGTTGCTGCCTGCACTG	7–5	EBP	T7/EBPR	56.5
2_4F	ACGCCATCCAGACGGAGGAT	2–4	NN	EBPF/SP6	58
4_6F	TCCTCCGACCCAGGTTGAA	4–6	NN	2_4F/C3R	59
T7	TAATACGACTCACTATAGGG	--	pcDNA3	2_4F/SP6	58.3
SP6	GATTTAGGTGACACTATAG	--	pcDNA3	4_6F/C3R	59
C1Fm	GAGACCCCATCGTGGCGCGA	1	m EBP/β-Gal	C1Fm/C1Rm	59
C1Rm	CTCTAGTAGCCAAGCAGGTAAGC	4	m β-Gal	EBPFm/EBPRm	58
EBPFm	GGGCTGAATGCTATCCAGATAC	2–5	m EBP
EBPRm	TGTGATATTGTTGCCTGCACGGT	7–5	m EBP

AT: annealing temperature; NN: not naturally ocurring; m:mouse.

^aGLB1 exon or exons that are complementary to the indicated primers