Skip to main content
. Author manuscript; available in PMC: 2010 Feb 1.
Published in final edited form as: Biochim Biophys Acta. 2008 Oct 7;1793(2):231–238. doi: 10.1016/j.bbamcr.2008.09.009

Figure 1.

Figure 1

EphA4 enhances apoptotic cell death that is blocked in a dose-dependent manner by ephrinB3. (a) HEK 293T cell cultures over-expressing EphA4 showed a higher number of dead cells as measured by Trypan blue exclusion. Addition of pre-clustered soluble ephrinB3 in the medium was able to reduce the amount of cells stained by Trypan blue. (b) EphA4 over-expression leads to an increase in TUNEL-positive cells 48 h after transfection. The number of TUNEL-positive cells is reduced upon addition of soluble ephrinB3 in the medium. (c) Representative pictures of TUNEL staining (quantification shown in (b)). The TUNEL-positive cells appear dark (a few are seen in the middle panel). (d) EphrinB3 blocked EphA4 induce cell death in a dose-dependent manner. The experiments were performed at least three times and the bars on the graphs represent the means of three separate wells (one representative experiment shown). * p<0.05; ** p<0.01; *** p<0.001.