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. Author manuscript; available in PMC: 2010 Feb 1.
Published in final edited form as: Biochim Biophys Acta. 2008 Oct 7;1793(2):231–238. doi: 10.1016/j.bbamcr.2008.09.009

Figure 2.

Figure 2

EphA4 induces caspase activation. (a) Caspase activity was increased as a result of EphA4 over-expression. This increase in caspase activity was reduced by adding ephrinB3 to the cell medium or by incubating the cells with the caspase inhibitor z-VAD-fmk or caspase-3 inhibitor zDEVD-fmk. Staurosporin was used as a positive control to induce apoptosis. The experiment was performed at least three times and the bars on the graphs represent the means of three separate wells (one representative experiment shown). (b) EphA4 cleavage fragments were observed 48 hours after over-expression. These small fragments (19–23 kDa) are no longer present upon addition of the caspase inhibitor z-VAD-fmk, supporting caspase cleavage of EphA4. **p<0.01.