Table 3.
Higher serum TGF-β1 protein on the day of vaccination results in lower IFN-γ ELISPOT responses to Ag85A peptides 4 months following vaccination with MVA85A alone
| Week 1 | Week 4 | Week 8 | Week 12 | Week 16 | Week 24 | Week 52 | |
|---|---|---|---|---|---|---|---|
| BCG | |||||||
| Correlation coefficient | −0.06 | 0.23 | −0.49 | 0.05 | ND | −0.03 | ND |
| p-Value (1 − tailed) | 0.457 | 0.329 | 0.160 | 0.467 | 0.478 | ||
| n | 6 | 6 | 6 | 5 | 6 | ||
| MVA85A | |||||||
| Correlation coefficient | −0.44 | −0.77 | −0.85 | −0.71 | −0.81 | 0.02 | ND |
| p-Value (1 − tailed) | 0.136 | 0.020 | 0.004 | 0.024 | 0.007 | 0.484 | |
| n | 8 | 7 | 8 | 8 | 8 | 7 | |
| BCG–MVA85A | |||||||
| Correlation coefficient | −0.07 | −0.10 | ND | −0.16 | ND | −0.53 | −0.57 |
| p-Value (1 − tailed) | 0.416 | 0.385 | 0.315 | 0.059 | 0.090 | ||
| n | 11 | 11 | 11 | 10 | 7 | ||
To determine if TGF-β1 protein at week 0 could effect long-term memory responses serum TGF-β1 was correlated (Spearman's correlation) with IFN-γ ELISPOT responses following vaccination with BCG alone, MVA85A alone and MVA85A vaccination of BCG primed subjects.