Figure 2.
5-HT2CRs in POMC Neurons Rescue the Attenuated Anorexigenic Effects of 5-HT Agents, Hyperphagia and Decreased Satiety in 2C Null Mice
Four-month-old body-weight-matched HFD-fed mice (n = 6–9 per genotype) were fasted overnight, and then saline, d-Fen (3 mg/kg) (A), or mCPP (5 mg/kg) (B) were intraperitoneally injected 30 min prior to presentation of HFD. Food intake in the following hour was measured and normalized by their body weight. (C) Weekly food intake was measured in singly housed HFD-fed mice (n = 5–7 per genotype) at 11 weeks. 2C null mice consumed significantly more diet than WT, 2C/POMC, and POMC-Cre mice, and there was no significant difference in food intake of WT, 2C/POMC, and POMC-Cre mice. Daily food intake (D), meal size (E), and meal frequency (F) of 3-month-old chow-fed mice (n = 5–11 per genotype) were measured with the TSE system. 2C null mice had significantly increased food intake and meal size than WT, and these parameters were not significantly different among WT, 2C/POMC, and POMC-Cre mice. There was no significant difference in meal frequency among the four genotypes. Data are presented as mean ± SEM, and ∗p < 0.05 and ∗∗p < 0.01 in one-way ANOVA analysis with Student-Newman-Keuls post hoc comparison.