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. Author manuscript; available in PMC: 2009 Aug 26.
Published in final edited form as: Neuroscience. 2008 Jun 19;155(3):597–602. doi: 10.1016/j.neuroscience.2008.06.027

Fig. 5. Buprenorphine concentration-dependently displaced specific binding of [3H]-OFQ/N in mouse brain homogenates.

Fig. 5

Wild-type mice (n = 3) were euthanized under isoflurane anesthesia, their brains removed and homogenized in Tris buffer (50 mM; pH = 7.4). Brain membrane homogenates were prepared (for details, see Method section) and assayed in triplicates for 3H-OFQ/N (5 nM) binding in the presence and absence of buprenorphine (0, 1, 10, 100 or 1000 nM). Non-specific binding was defined in the presence of non-labeled OFQ/N (5 µM). *indicates a significant increase as compared to control (p<0.05). †indicates a significant increase as compared to all other groups (p<0.05).