Figure 3. PKCα regulates dense-granule but not α-granule biogenesis.

Washed platelets from WT or Prkca^–/– mice were examined by transmission electron microscopy, and the number of dense granules (black arrows) and α-granules (white arrows) was quantified as described in Methods. (A) Images of WT (left panel) or Prkca^–/– (right panel) platelets are representative of 3 independent experiments. Scale bar: 1 μm. Original magnification, ×19,000. (B) Dense granules (left panel) and α-granules (right panel) were counted per field of view (25–30 fields of view per preparation) and shown as mean ± SEM for number of granules per μm². n = 3. *P < 0.05.