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. 2009 Jan 26;119(2):305–314. doi: 10.1172/JCI35958

Figure 5. Aspirin inhibits the Nalp3 pathway and reduces APAP-induced liver injury.

Figure 5

(A) Nalp3+/+ or Nalp3–/– mice were injected with MSU crystals i.p. (3 mg/mouse). After 3 hours, peritoneal lavage was performed and the number of GR-1–positive neutrophils quantified (*P < 0.0001). (B) Wild-type mice were treated with or without aspirin (60 mg/l) in the drinking water for 3 days and then injected i.p. with MSU crystals or PBS. After 3 hours, peritoneal lavage was performed and the number of Gr-1–positive neutrophils quantified (*P < 0.0001). (C) Survival analysis of mice treated with and without aspirin (60 mg/l) in the drinking water for 3 days and then injected i.p. with APAP (500 mg/kg) (control drinking water: n = 13, aspirin drinking water: n = 17, P < 0.02). (D) H&E-stained liver tissue sections (original magnification, ×20) from wild-type mice 12 hours after i.p. injection with APAP or PBS. Mice were on aspirin or regular drinking water for 3 days prior to APAP injection. There is substantial reduction in APAP-induced liver injury and hemorrhage in mice receiving aspirin. (E) ALT levels in serum from wild-type mice 12 hours after APAP injection with and without pretreatment with aspirin. The aspirin-treated group had significantly lower serum ALT levels (P < 0.04). Error bars indicate 1 SD.