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. 2009 Jan;174(1):101–114. doi: 10.2353/ajpath.2009.080599

Figure 8.

Figure 8

c-Jun protein and DNA-binding are elevated in dermal fibroblasts cultured in MMP-1-fragmented three-dimensional collagen lattices. Human dermal fibroblasts were cultured in intact or MMP-1-fragmented lattices. A: c-Jun protein levels were determined by laser confocal immunofluorescence microscopy and quantified by image analysis. Top Panels show representative images. Cell nuclei are stained blue, and c-Jun protein is stained red. Results are means ± SEM. n = 3, *P < 0.05. B: Nuclear extracts were prepared and c-Jun DNA binding was determined using the TransAM AP-1 kit (Active Motif). Results are means ± SEM. n = 3, *P < 0.05. C: α2 and β1 integrins are elevated in dermal fibroblasts cultured in MMP-1-fragmented three-dimensional collagen lattices. Human dermal fibroblasts were cultured in intact and MMP-1-fragmented three-dimensional collagen lattices. Total RNA was extracted. α2 and β1 integrin mRNA levels were analyzed by real-time RT-PCR (10 ng total RNA). Results are mean ± SEM of α2 or β1 integrin mRNA normalized to 36B4 (internal control) mRNA. n = 3, *P < 0.03.