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. 2009 Jan;174(1):177–183. doi: 10.2353/ajpath.2009.080342

Figure 4.

Figure 4

Co-localization and density of AnxA1 and Fpr2 immunogold particles on the plasma membrane of mesenteric and peritoneal neutrophils. Co-localized particles of AnxA1 and Fpr2 receptor (10-nm and 20-nm colloidal gold particles, respectively) were detected on plasma membrane (arrowheads) of neutrophils, from mesentery and peritoneal fluid, after 4 hours of carrageenin induction (A, B) and the peptide Ac2-26 treatment (C, D). E: Absence of gold labeling for AnxA1 and Fpr2 in neutrophils incubated with nonimmune sheep and rabbit serum. N, nucleus. F: Statistical analysis. Data are mean ± SEM of gold particles per μm of 10 distinct neutrophils analyzed at 4 hours after carrageenin-induced peritonitis and after peptide Ac2-26 treatment. *P < 0.05, **P < 0.01 and ***P < 0.001 versus CG4h group. Analysis of variance plus Bonferroni test. Scale bars = 0.5 μm.