Figure 8.

Endogenous 3R mouse tau adopts disease-associated properties in rTg4510 mice. A: Immunoprecipitation (IP) with RD3 antibody of soluble tau in 1M rTg4510 and non-transgenic littermates followed by detection with phospho-tau antibodies (indicated on left) showed that rTg4510 mice had higher levels of phosphorylated 3R tau than non-transgenic mice. Inputs indicate whole brain homogenate. Optical densitometry for rTg4510 mice as a percentage of the non-transgenic mice is shown for all tau species analyzed in the RD3 IP lanes. B: IP with Alz50, an IgM that recognizes a specific disease-associated tau conformation, from the same samples followed by RD3 detection using IgG showed that more 3R tau was also in the Alz50 conformation in rTg4510 mice compared with non-transgenics. Optical densitometry for rTg4510 and non-transgenic mice is shown for RD3 in the Alz50 IP lanes.