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. 2009 Jan;174(1):265–275. doi: 10.2353/ajpath.2009.071006

Figure 1.

Figure 1

AP-1 promoter activity in K12 and K7M2 osteosarcoma cell lines. AP-1 promoter activity was assayed by transient transfection of TRE2-Luciferase containing two AP-1 binding sites (A) and 4×AP-1-luciferase containing four AP-1 binding sites (B) and the −1048 to + 205 cyclin A promoter (C) fused to the luciferase reporter gene. Results are representative of the mean ± SD of experiments performed in triplicate. Renilla-luciferase under the control of the TK promoter was used as a control for transfection efficiency. Empty vectors and 4×mut AP-1-Luciferase containing four mutated AP-1 binding sites were used as negative controls (*P < 0.05, n = 4).