Skip to main content
. 2009 Jan;174(1):265–275. doi: 10.2353/ajpath.2009.071006

Figure 5.

Figure 5

Inducible dominant-negative cJun expression in K7M2 cells. A: K7M2 clones with stable inducible expression of GFP or a dominant-negative cJun, Tam67, were prepared as described in Materials and Methods. Inducible expression of Tam67 was determined by Western blot analysis using a cJun-specific antibody that recognizes Tam67 as a 29kD protein. Clone K7M2-Tam67–3 had the highest inducible Tam67 expression. B: Western blots showing a time course of doxycycline-inducible Tam67 expression in K7M2-Tam67 cells. Inducible Tam67 expression results in the inhibition of endogenous cJun expression. Results are representative of at least three independent clones. Tubulin is shown as a control for differences in loading. C: The effect of inducible Tam67 on the growth of K7M2 cells. Cell growth in the absence and presence of 2 μg/ml doxycycline (+Tam67) was performed in 96 well plates and measured by MTT assays (Promega) as described in materials and methods. Results are the mean ± SD of eight wells per condition. The doubling time for K7M2 cells was not significantly changed by TAM67.