Figure 6.

AP-1 promoter activity is inhibited by inducible Tam67 expression. K7M2-GFP and K7M2-Tam67 cells grown in the absence and presence of doxycycline for 24 hours were transiently transfected with the TRE₂-promoter-luciferase construct containing two AP-1 binding sites and lysates extracted after 48 hour incubations. A: Luciferase activity was inhibited by inducible Tam67 expression in K7M2-Tam67 cells and not in K7M2-GFP control cells. (*P < 0.05, n = 3). B: Tam67 expression had an inhibitory effect Renilla-luciferase expression, although not to the same extent as that on the TRE₂-Promoter construct. Results are the mean ± SD of experiments performed in triplicate.