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. 2009 Jan;174(1):276–286. doi: 10.2353/ajpath.2009.080086

Figure 2.

Figure 2

Deletion of Irs2 suppresses solid tumor invasion in Pten+/− mice. A: Invasive neoplasms of prostate (a), endometrium with vascular invasion (c, arrow), colon (e), and breast (g) were observed in Pten+/− mice when Irs2 was wild type, whereas the neoplasms of the same organs, prostate (b), endometrium (d), colon (f), and breast (h) remained in situ, ie, did not expand beyond preformed glandular structures, in Pten+/−Irs2−/− mice. (N.B.: a single intraductal papilloma was noted among 10 Pten+/−Irs2−/− female mice) B and C: The extent of PIN (B) and EIN (C) in Pten+/− mice, measured as percentage of glands involved by PIN or EIN versus total number of glandular profiles, was significantly decreased when both Irs2 alleles were deleted in the 150- to 330-day study period [Pten+/−Irs2+/+ and Pten+/−Irs2−/− males (n = 9 and 9, respectively) and for Pten+/−Irs2+/+ and Pten+/−Irs2−/− females (n = 14 and 10, respectively)]. For the 150- to 400-day study period [Pten+/−Irs2+/+ and Pten+/−Irs2+/− males (n = 30 and 14, respectively) and for Pten+/−Irs2+/+ and Pten+/−Irs2+/− females (n = 17 and 29, respectively)] both extent of PIN (B) and EIN (C) were reduced in Pten+/−Irs2+/− versus Pten+/−Irs2+/+ mice but this was only statistically significant (P < 0.05) for EIN. D: Irs2 deletion suppresses adrenal medulla growth in males and females because of Pten+/−. In adrenal glands medulla to cortex ratio (M/C ratio) was significantly reduced (P < 0.05) in Pten+/−Irs2−/− but not in Pten+/−Irs2+/− mice versus Pten+/−Irs2+/+ controls. Original magnifications: ×200 (a, b, c, e); ×100 (d, f, g, h).