Figure 1.

Loss of HP1γ is required for adipocyte differentiation. A: Western blot analysis of HP1α, β, and γ in 3T3L1 and human preadipocyte cells during adipogenesis. GAPDH was also examined as a loading control. B: Western blot analysis of HP1γ in 3T3L1 cells (wt) and 3T3L1-derived cells with a mifepristone-inducible HP1γ expression system (tr). The cells were treated with or without mifepristone and/or the differentiation reagents (3-isobutyl-1-methylxanthine, dexamethasone, and insulin). GAPDH was used as a loading control. C: Oil red staining of 3T3L1 cells (wild) and 3T3L1-derived cells with a mifepristone-inducible HP1γ-full length, -ΔCSD, or -ΔCD expression system (transfectant) during adipogenesis. The cells were treated with mifepristone and/or the differentiation reagents. Lipid duplets in adipose are visualized as red stains.