Figure 2.

The effect of GSK-3 deficiency on glucosamine-induced lipid accumulation. Wild-type, GSK-3α^−/−, and GSK-3β^−/− MEFs were challenged with 0 or 5 mmol/L glucosamine for 18 hours. Cells were stained with filipin and fluorescence was examined by FACS (A) and fluorescence microscopy (B). For FACS, >10,000 cells were analyzed from each treatment group using excitation wavelengths of 350 to 370 nm and emission detection at 420 to 460 nm.