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Effect of valproate supplementation on hepatic GSK-3 activity. GSK-3β was immunoprecipitated from protein lysates prepared from the livers of normoglycemic (control) and hyperglycemic (HG) mice fed control or valproate-supplemented diet. GSK-3β kinase activity was determined as previously described.10 n = 3, *P < 0.05 relative to nonsupplemented control. **P < 0.05 relative to nonsupplemented HG.
