Figure 2.

Time Course of Regulation of Gene Expression by E2 or EDC

MCF-7 cells were treated with 0.1% EtOH vehicle, 10 nm E2 (solid line), empty dendrimer or 10 nm estrogen equivalent EDC (dotted line) for 2, 4, and 8 h, and mRNA levels were monitored by Q-PCR. mRNA levels were normalized relative to 36B4, and fold change was calculated relative to control. Results are the average ± sd of at least three independent experiments.