Figure 6.

GR Interacts with Sp3 Transcription Factor

Cardiomyocytes were treated with vehicle or 1 μm CT for 4 h. Nuclear proteins were harvested for measurements of GR protein level by Western blot (upper panel) or for immunoprecipitation using mouse IgG control, BuGR2 monoclonal antibody, or Sp3 antibody. GR or Sp3 protein in the immunocomplex was determined by Western blot with polyclonal antibodies against GR or Sp3. The same set of membrane was blotted with Sp3 antibody and then GR antibody after stripping (left panel) or vice versa (right panel). The data represent one of three independent experiments. ctr, Control; IP, immunoprecipitation.