Figure 11.
Other Gene Expression Changes in Preantral Follicles from Control and Rb cKO Females
Real-time PCR analysis of mRNA from WT and Rb cKO (Rbflox/−, Amhr2Cre+) preantral follicles isolated from 12- d-old females. Preantral follicles were collected from three independent control and Rb cKO females. Average and sem are shown. A significant decrease was seen in the relative quantity (RQ) of Foxl2 (A), Cyp19a1 (B), and Nr5a1/Sf1(C), whereas a significant increase was observed in the levels of Nr5a2/Lrh-1 (D), Nr0b1/Dax-1 (E), and Fos (F) in Rb cKO preantral follicles. The value of one of the control samples was set to equal 1. *, P < 0.05.