Figure 8.

FSHR and FSH Target Gene Expression Changes in Prepubertal Ovaries and Preantral Follicles from Control and Rb cKO Females

Real-time PCR analysis of mRNA from 2- and 3-wk-old control (WT and Rb^flox/−) and Rb cKO (Rb^flox/−, Amhr2Cre⁺) ovaries and preantral follicles isolated from 12-d-old females. Ovaries and preantral follicles were collected from three to four independent control and Rb cKO females. Average and sem are shown. A significant increase was seen in the relative quantity (RQ) of Fshr (A and B), Ccnd2 (D and E), and Inha (E and F) in Rb cKO ovaries and preantral follicles. The value of one of the control samples was set to equal 1. *, P < 0.05.