Figure 5.
Proper DNA Binding Is Required for SUMO-Mediated Synergy Control
A, Schematic representation of the Gal4-responsive promoter showing the sequences of either the WT consensus Gal4 site (top), or the mutated response elements harboring a G to A substitution (bottom). Key bases contacted by the Gal4 DBD are in bold. The diagram of the AF-1 Gal DBD fusion is shown above. B, CV-1 cells were transfected with WT or mutant pΔ(Gal4)2-Luc promoters along with AF-1 Gal4DBD fusions with either intact or disrupted SC motifs or an equimolar amount of empty expression vector. Data represent averages ± sem of three to four independent transfections performed in triplicate and are expressed as a percentage of the WT AF-1 Gal DBD activity at the WT (Gal4)2 promoter (10.0 ± 2.0). Mut, Mutant; RE, response element; Vect, vector.