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. Author manuscript; available in PMC: 2009 Jan 27.
Published in final edited form as: Biochemistry. 2006 Sep 26;45(38):11642–11649. doi: 10.1021/bi060980l

Figure 2.

Figure 2

Time course of Heme-Transfer from PhuS to pa-HO as a function of pH. (– –) pH 6.5; (—) 7.5; (····) 8.5; (–··–) pa-HO-G125V, pH 7.5; and (–·–) pa-HO-Wt with 10mM KCN, pH 7.5. Experiments were conducted with 10μM PhuS, and 30μM pa-HO in 20mM Tris-HCl at the corresponding pH at 25 °C, and time course were measured at 405 (for pH 6.5 and 7.5) and 410nm (pH 8.5). Reaction conducted in the presence of KCN was monitored at 419nm.