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. Author manuscript; available in PMC: 2009 Jan 27.
Published in final edited form as: Mol Cell. 2005 Nov 23;20(4):613–622. doi: 10.1016/j.molcel.2005.10.007

Figure 4. Effects of 23S rRNA Mutations C2394A, G2252U, and G2553U on Reading-Frame Retention after Translocation of Several Forms of tRNATyr2.

Figure 4

Pretranslocation complexes were made by incubating ribosomes with m299 and tRNAfMet to fill the P site (P lanes) and then adding N-acetyl-Tyr-tRNATyr2, Tyr-tRNATyr2, tRNATyr2, or tRNATyr2Δ3′ to bind the A site (A lanes), as indicated. Complexes were then incubated in the presence of GTP (− lanes) or EF-G plus GTP (+ lanes). At each stage of the experiment, the position of mRNA was mapped by toeprinting.