Table 1.
Cofactor and co-substrate requirement for SgcC3-catalyzed chlorination of (S)-β-tyrosyl-S-SgcC2 (6) in vitro.
Entrya | Condition | Conversion | Relative rate |
---|---|---|---|
II | Complete assay | 38% | 100 |
III | Complete assay – SgcE6 + E. Coli Fre | 39% | 100 |
IV | Complete assay – SgcE6 | 6% | 16 |
V | Complete assay – FAD | 11% | 30 |
VI | Complete assay – NADH | 0 | 0 |
VII | Complete assay – O2 | 0 | 0 |
Entry number corresponds to the chromatogram shown in Figure 5B.