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. Author manuscript; available in PMC: 2009 Sep 1.
Published in final edited form as: J Neurochem. 2008 Jul 4;106(5):2236–2248. doi: 10.1111/j.1471-4159.2008.05551.x

Figure 4.

Figure 4

DAPT affects distribution of high molecular weight neurofilament (NF-H) proteins. E18 rat embryonic cortical neurons were cultured for 7 d in B27/neurobasal medium and then treated with either DMSO (a-d) or 10 μM DAPT (e-h) for 24 h. Cells were lysed for immunoblotting or fixed for immunocytochemistry (ICC). (A) Fixed cells were immunostained for phospho-NF-H using R97 antibody (a, e) and total NF-H using anti-NF-H antibody (b, f). Nuclei are stained with DAPI (c, g). Merged image of p-NF-H, total NF-H and DAPI staining of DMSO-treated cells is shown (d) and that DAPT-treated cells (h) are shown. (B) Immunoblot shows p-NF-H and total NF-H levels in the DMSO- and DAPT-treated neurons.