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. Author manuscript; available in PMC: 2009 Sep 1.
Published in final edited form as: J Neurochem. 2008 Jul 4;106(5):2236–2248. doi: 10.1111/j.1471-4159.2008.05551.x

Figure 7.

Figure 7

DAPT does not disrupt cdk5/p35 interaction. (A) In neurons after 24 h of DAPT treatment, cdk5 colocalizes with p35 comparably (indicated by arrows) in the control, DMSO-treated (a-c) and DAPT-treated cells (d-f). (B) Neurons were treated with DMSO or DAPT for 24 h. Cell lysates were prepared and one milligram of total protein from each sample was immunoprecipitated using the anti-cdk5 antibody. The control groups included lysates with no antibody for immunoprecipitation. The immunoprecipitates were resolved in an SDS-PAGE gel, immunoblotted for detection of p35 using an anti-p35 antibody or anti-cdk5 antibody.