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. Author manuscript; available in PMC: 2009 Jan 27.
Published in final edited form as: Biomark Med. 2007 Jun;1(1):159–185. doi: 10.2217/17520363.1.1.159

Figure 5. Reproducibility of dual-column capillary LC-MS analyses of Cyanothece sp. ATCC 51142 metabolite extract.

Figure 5

An in-house constructed dual-column capillary LC system was coupled with an LTQ-Orbitrap MS and utilized in replicate analyses of the same Cyanothece metabolite extract. Five replicates of the same sample were analyzed on both columns, and nine datasets were used for comparative analyses (Column 1, Rep D was excluded from the data analysis due to the presence of air bubbles during injection). The upper panel illustrates the agreement between intensity measurements for individual features in the aligned and baseline datasets. The lower panel illustrates agreement between intensity measurements in the aligned and baseline datasets, in terms of an intensity ratio histogram. The dataset corresponding to Column 1, Rep A was arbitrarily selected as a baseline for both chromatographic alignment and intensity normalization. Unpublished data.