Figure 7.
Growth phase dependent activation of putative Mtb whiB1 promoter in M. smegmatis mc2155. M. smegmatis cells carrying pJEM15 (vector control, white bar) or pwhiB1-lacZ (grey bar) were aerobically grown for different periods as indicated. Cells were aliquoted at different time points (indicated as h, at the bottom of each bar) and A600 was measured followed by the preparation of whole cell lysate. The β-gal activity was measured in standard 'Z' buffer. Inset: Growth curve of M. smegmatis cells.