Table 1.
Primer | Sequence |
---|---|
P1 | 5'-CCTCTTCTCTTGTTGccATGGAG-3' |
P2 | 5'-CAGTACAGCAAATTAccaTGGGTTTG-3' |
P3 | 5'-TTTCCCTGTCTTATTGCcATGGAA-3' |
P4 | 5'-CCAGTACAGCATATTAccaTGGGTTTA-3' |
P5 | 5'-CTGCTTCAAACtgtACaTAATTTGC-3' |
P6 | 5'-GAAAGTAAAATgcGGccGCTCATCC-3' |
P7 | 5'-TTGTTTCTGAAACTTGGTTACAG-3' |
P8 | 5'-TTACTTCTTTTGCTTCTTCCCTGGCTT-3' |
P9 | 5'-AATGG(A/C)AC(C/T)G(A/G)(A/T)ATGGTCAAG-3' |
P10 | 5'-TTAGAAGCA(C/T)TTC(A/C)TGTG(C/G)AC-3' |
Underline: restriction sites for BsrGI, NcoI and NotI.
Lowercase letters: deviations from the original OsRpl6-1 sequence to enable introduction of restriction sites.
The locations of primers P1-P8 are shown in Figure 1A.
Primers P9 and P10 were designed from rice Actin sequences [39] as described previously [40] with slight modifications.