Table 1.
Microsatellite loci assayed from adult Gobiusculus flavescens.
| Locus | Primer sequence (5'-3') | Repeat Motif | Temp (°C) | N | A | HO | HE | Excl. prob |
| 2SG-08 | F: TGATGGTTCTTCTTTCAATATGC R: GCTGCTGGACACCTGAATTT |
(GATA)13 | 58 | 33 | 18 | 0.848 | 0.908 | 0.811 |
| 2SG-17 | F: GCTGCTGGACACCTGAATTT R: CGATCGCCTTTCAGTTTGAC |
(CTAT)11 | 56 | 33 | 15 | 0.788 | 0.763 | 0.592 |
| 2SG-21 | F: TGTAGGTGCCTTCCCCATTA R: GGACTCCTGCATCTCTGCAT |
(GATA)10 | 58 | 33 | 16 | 0.758 | 0.842 | 0.685 |
| 2SG-55 | F: CATACATGCGTGCTCAAAAA R: TGTCGGTATTGAAACATCCAA |
(NTAT)14 a | 59 | 31 | 13 | 0.839 | 0.821 | 0.650 |
aActual sequence = (CTAT)4GTAT(CTAT)2(GTATCTAT)2(CTAT)3
Name of locus, primer sequence of the original cloned microsatellite, PCR reannealing temperature (°C), number of adults assayed (N), number of alleles per locus (A), observed (HO) and expected heterozygosity (HE) and exclusion probabilities (Excl. prob; given one parent known with certainty and the second parent unknown) are listed for each locus.