Figure 3.

Effects of monocytes in potentiating the effects of IGF-I on PHA-induced changes in CD45 isoform expression in purified CD3+ T cell culture. CD3+ T cells were incubated with PHA (1 μg/ml) alone, PHA + IGF-I (100 ng/ml), PHA + autologous CD14+ monocytes (monocytes: T cells ratio = 1:4), or PHA + IGF-I + monocytes for 6 days. The addition of monocytes promoted CD4+ and CD8+ T cells maturation and the effect was further increased in the presence of IGF-I. Results shown are from 7 independent cases of CD3+ T cells respectively.