. Author manuscript; available in PMC: 2009 Nov 15.

Published in final edited form as: Bioorg Med Chem Lett. 2008 Jul 20;18(22):5867–5870. doi: 10.1016/j.bmcl.2008.07.068

Table 2.

Cell cycle and apoptosis analysis of Vero cells treated with colchicine, colchicine-504 and colchicine-646.

Compound	EC₅₀^a,^b(µM) [Apoptosis]	EC₅₀^a,^b(µM) [Cell cycle arrest]
Colchicine	0.39 ±0.13	0.27 ±0.09
504	2.6 ±0.7	0.96 ±0.28
646	10.5 ±2.3	6.8 ±3.1

Vero cells were treated with compounds in a dose-response manner and analyzed after 18 hours.

Cells were stained with propidium iodide (PI) using a CycleTEST Plus DNA reagent kit (Beckton Dickinson). EC₅₀ values were determined by cell-cycle analysis representing the concentration for 50% cells arrested in G2/M phase. The EC₅₀ values were obtained by fitting data to the following equation (y = min + (max − min)/1 + (x/Kd) Hill slope). Values are means of two independent experiments.

Cells were treated with Caspase-Glo (Promega) and measured after 1 hour. The EC₅₀ values were obtained by fitting data to the following equation (y = min + (max − min)/1 + (x/Kd) Hill slope). Values are means of two independent experiments carried out in duplicate.