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. Author manuscript; available in PMC: 2009 Nov 15.
Published in final edited form as: Exp Cell Res. 2008 Sep 20;314(19):3551–3562. doi: 10.1016/j.yexcr.2008.09.006

Fig. 2. Integrity of epithelial polarity in SYT expressants.

Fig. 2

Confocal microscopy of MDCK cells infected with WT SYT, the SYT deletion (dl) mutants, and grown in collagen for 7 days. (A) Cysts were double stained with antibodies to the apical Golgi protein GM130 (red) and with anti-HA antibody (to visualize the expressed SYT cDNAs). Polarization was also assessed by the luminal localization of an actin ring (red) at the apical surface, the basolateral marker β-catenin (green) and the apical marker ZO-1 (red). (B) Cellular levels of polarity markers in the monolayers of SYT dl mutants-expressing MDCK cells.