. Author manuscript; available in PMC: 2009 Nov 15.

Published in final edited form as: Exp Cell Res. 2008 Sep 20;314(19):3551–3562. doi: 10.1016/j.yexcr.2008.09.006

Table 1.

Effect of signaling inhibitors on MDCK cyst formation

			Effect on cyst formation
Inhibitor	Conc.(μM)	Pathway affected	WT SYT	SYTdl 1–40
Untreated	-----	-----	None	None
DMSO	-----	-----	None	None
Genestein	40	Tyrosine kinase CFTR	Complete inhibition	Complete inhibition
AG1478	10	EGFR	None	None
Reactive Blue 2	500	P2YR	Complete inhibition	Complete inhibition
PPADS	500	P2YR	Profound inhibition^†	Profound inhibition
NF023	50	P2XR	None	None
LY290042	10	PI3K	Reduced Growth^a	Reduced Growth
H-89	10	PKA	None	None
Calphostin	2.5	PKC	None	None
W-7	50	Calcium	None	None
PD98069	50	MEK1	Complete inhibition	Complete inhibition
SP600125	50	JNK	Complete inhibition	Complete inhibition
SB220025	50	p38MAPK	Complete inhibition	Complete inhibition
Rac Inhibitor	50	Rac1	Complete inhibition	Complete inhibition

reduced growth signifies that the size of the individual MDCK cysts was around half the size of the untreated control. Bolded names designate P2YR specific inhibitors.

^†

Profound inhibition implies a very significant reduction in the number of intact cysts caused by the PPADS inhibitor. The reduction is quantitated in Table 2.